Description
General Description
The Crystal Violet Cell Viability Assay Kit can be used as a quick and easy assay to assess cell viability after experimental treatments. Crystal Violet (CV), a triarylmethane dye, binds to DNA in the cell nucleus and is therefore proportionate to cell biomass. Dead cells in the culture will detach and be removed from the plate during the washing steps, and only viable adherent cells will remain attached to the plate. The optical density (O.D.) of CV measured at 570nm after solubilization of stained cells is used to indirectly quantify the relative density of viable cells in each well, and thus is used to determine cell viability. This kit produces results which are sensitive, accurate, and reproducible. The CV Cell Viability Assay Kit is also a simple and effective assay for cytotoxicity studies and for drug screening using adherent cell cultures.
Features
- Simple and effective assay for in vitro:
- Cell viability studies
- Cytotoxicity studies
- High-throughput drug screening
- Detection using absorbance (O.D.) at 570 nm
- Sample type – adherent cell culture (animal)
- Quick and easy assay; less than 2 hours
- Sensitive and reproducible
- Available with 96-, 48-, or 24-well plates (2 each)
- Requires: 100% ethanol, microplate reader, orbital shaker
