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DMEM/F-12 Medium with L-glutamine, no phenol red, no HEPES

Original price was: $41.75.Current price is: $31.75.

Dulbecco’s Modified Eagle Medium: Ham’s F-12 Nutrient Mixture (DMEM/F-12) with L-glutamine, no phenol red, no HEPES is a widely used basal culture medium formulated for the growth of mammalian cells

[+] L-glutamine; [-] phenol red; [-] HEPES

500 mL

Dulbecco’s Modified Eagle Medium: Ham’s F-12 Nutrient Mixture (DMEM/F-12) with L-glutamine, no phenol red, no HEPES by JangoCell is a sterile, serum-free basal cell culture medium with added L-glutamine. This cell culture media is formulated for the growth of a variety of mammalian cell types including stem cells, glial cells, fibroblasts, macrophages and endothelial cells. In addition, DMEM/F-12 media has been used across a broad range of cell culture applications and experimental studies in neuroscience, carcinogenesis, pharmacokinetics, gene expression, and regenerative medicine.

This 1:1 mixture combines the diverse nutrient mixture of Ham’s F-12 culture media with the high glucose, amino acids, and vitamins of DMEM media. DMEM/F-12 liquid culture media contains no growth factors, proteins or lipids (animal origin-free), so supplementation of these factors may be required for your specific cell type; 10% Fetal Bovine Serum (FBS) is often used. JangoCell’s DMEM/F-12 is manufactured under strict industry standards.

Phenol Red is known to interfere with certain biochemical reactions and fluorescent dyes. Phenol red free media circumvents these issues, allowing no confusion with data interpretation.

Dulbecco’s Modified Eagle Medium / Ham’s F-12 Nutrient Mixture (DMEM/F-12) is also known as: Dulbecco’s Modified Eagle’s Medium/Ham’s F-12, DMEM/F12, DMEM F12, DMEM/Ham’s F-12, (DMEM)/F12, DMEM-Ham’s F12, and DMEM: F-12.

 

This DMEM/F-12 is modified as follows:

[+] L-glutamine

[-] Phenol red

[-] HEPES

 

Product Use

This product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.

Catalog Number

104020

Country of Origin

USA

Lot Number

Included with shipment

Intended Use

Mammalian cell types; stem cells, glial cells, fibroblasts, macrophages and endothelial cells

Classification

Animal Origin-free

Concentration

1X

Details

[+] L-glutamine, [-] phenol red, [-] HEPES

Volume

500 mL

Packaging

Bottles

Form

Liquid

Glutamine

L-glutamine

HEPES Buffer

No HEPES

Phenol Red Indicator

Without Phenol Red

Osmolality

305 – 330 mM/Kg

pH

7.0 – 7.4

Endotoxin

≤ 0.1 EU/mL

Mycoplasma

Negative

Sterility

Sterile

Storage

Refrigerate at 2°C – 8°C (protect from light)

Shelf-Life

18 months from date of manufacture

Shipping Conditions

Ambient temperature; Ground Shipping. Refrigerate on arrival

Also Known As

Dulbecco’s Modified Eagle’s Medium/Ham’s F-12, DMEM/F12, DMEM F12, DMEM/Ham’s F-12, (DMEM)/F12, DMEM-Ham’s F12, and DMEM: F-12

Product Use

This product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.

References

Palmer TD, Markakis EA, Willhoite AR, Safar F, and Gage FH. Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS. The Journal of Neuroscience. 1999; 19 (19): 8487-8497. doi: 10.1523/JNEUROSCI.19-19-08487.1999.

Tang B, Vu M, Booker T, Santner SJ, Miller FR, Anver MR, and Wakefield LM. TGF-beta switches from tumor suppressor to prometastatic factor in a model of breast cancer progression. Journal of Clinical Investigation. 2003; 112 (7): 1116-1124. doi: 10.1172/JCI18899.

De La Cruz-Rivera PC, Kanchwala M, Liang H, Kumar A, Wang LF, Xing C, and Schoggins JW. The IFN response in bats displays distinctive IFN-stimulated gene expression kinetics with atypical RNASEL induction. The Journal of Immunology. 2018; 200 (1): 209-217. doi: 10.4049/jimmunol.1701214.

Morgan A, Babu D, Reiz B, et al. Caution for the routine use of phenol red – It is more than just a pH indicator. Chemico-Biological Interactions. 2019; 310: 108739. doi: 10.1016/j.cbi.2019.108739.

Nam K, Kim K, Dean SM, Brown CT, Davis RS, Okano T, and Baker OJ. Using cell sheets to regenerate mouse submandibular glands. npj Regenerative Medicine. 2019; 4: 16. doi: 10.1038/s41536-019-0078-3.

Contact us for the technical documents on this product.

Contact us for the formulation.

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