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DMEM/F-12 Medium with L-glutamine, no phenol red, no HEPES


Dulbecco’s Modified Eagle Medium: Ham’s F-12 Nutrient Mixture (DMEM/F-12) with L-glutamine, no phenol red, no HEPES is a widely used basal medium formulated for the growth of mammalian cells

[+] L-glutamine; [-] phenol red; [-] HEPES

500 mL

Dulbecco’s Modified Eagle Medium: Ham’s F-12 Nutrient Mixture (DMEM/F-12) with L-glutamine, no phenol red, no HEPES by JangoCell is a sterile, serum-free basal medium with added L-glutamine. This media is formulated for the growth of a variety of mammalian cell types including stem cells, glial cells, fibroblasts, macrophages and endothelial cells. In addition, DMEM/F-12 has been used across a broad range of cell culture applications and experimental studies in neuroscience, carcinogenesis, pharmacokinetics, gene expression, and regenerative medicine.

This 1:1 mixture combines the diverse nutrient mixture of Ham’s F-12 with the high glucose, amino acids, and vitamins of DMEM. DMEM/F-12 contains no growth factors, proteins or lipids, so supplementation of these factors may be required for your specific cell type; 10% Fetal Bovine Serum (FBS) is often used.

Phenol Red is known to interfere with certain biochemical reactions. Phenol-red free media circumvents these issues, allowing certainty with data interpretation.


Catalog Number 104020
Lot Number Included with Shipment
Details [+] L-glutamine, no phenol red, no HEPES
Country of Manufacture United States
Quantity 500 mL each
Storage Refrigerate at 2-8˚C
Shipping Info Room temperature, ground shipping, refrigerate on arrival.
Product Use Not for Human or Animal Therapeutic Use. Uses other than the labeled intended use may be a violation of federal law.
References Palmer TD, Markakis EA, Willhoite AR, Safar F, and Gage FH. Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS. The Journal of Neuroscience. 1999; 19 (19): 8487-8497. doi: 10.1523/JNEUROSCI.19-19-08487.1999.

Tang B, Vu M, Booker T, Santner SJ, Miller FR, Anver MR, and Wakefield LM. TGF-beta switches from tumor suppressor to prometastatic factor in a model of breast cancer progression. Journal of Clinical Investigation. 2003; 112 (7): 1116-1124. doi: 10.1172/JCI18899.

De La Cruz-Rivera PC, Kanchwala M, Liang H, Kumar A, Wang LF, Xing C, and Schoggins JW. The IFN response in bats displays distinctive IFN-stimulated gene expression kinetics with atypical RNASEL induction. The Journal of Immunology. 2018; 200 (1): 209-217. doi: 10.4049/jimmunol.1701214.

Nam K, Kim K, Dean SM, Brown CT, Davis RS, Okano T, and Baker OJ. Using cell sheets to regenerate mouse submandibular glands. npj Regenerative Medicine. 2019; 4: 16. doi: 10.1038/s41536-019-0078-3.

Technical Resources


Certificate of Analysis – Basal Media


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