DMEM/F12 Medium with L-glutamine and HEPES, no phenol red, by Captivate Bio (case of 6 L)

DMEM/F12 Medium with L-glutamine and HEPES, and added sodium pyruvate, no phenol red, by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. DMEM/F12 medium is a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. Cells cultured in DMEM/F12 with HEPES include mouse embryonic stem cells, epithelial cells, human endothelial cells, neural stem cells, human embryonic stem cells, mesenchymal stem cells, induced pluripotent stem cells, and hematopoietic stem cells.

DMEM/F12 is a serum-free, basal cell culture medium that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

This DMEM/F12 is modified as follows:

[+] L-Glutamine

[+] HEPES

[+] Sodium Pyruvate

[-] Phenol Red

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.

Specifications

Catalog Number	CB-DFHG02-CS6
Manufacturer / Country of Origin	Captivate Bio / United States
Lot Number	Included with shipment
Intended Use	Mammalian cell culture, hPSCs, fibroblasts, human, mouse, rat, non-human primate, other cells
Details	[+] L-Glutamine, [+] HEPES, [+] Sodium Pyruvate, [-] Phenol Red
Quantity	6 / case
Volume	1000 mL
Packaging	Bottles
Form	Liquid
Glutamine	L-Glutamine
HEPES Buffer	With HEPES
Phenol Red Indicator	No Phenol Red
Osmolality	280-305 mOsm/kg
pH	7.0-7.4
Endotoxin	< 1.0 EU/mL
Mycoplasma	Negative
Sterility	Sterile
Storage	2°C – 8°C (protect from light)
Shelf-Life	12 months from date of manufacture
Shipping Conditions	Ambient temperature
Quality Statement	This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.
Research	Morton, H.J. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89-108. doi: 10.1007/BF02616112
	Ham, R.G. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci. 1965;53(2):288-293. doi: 10.1073/pnas.53.2.288
	Smith, J.D., Freeman, G., Vogt, M., and Dulbecco, R. The nucleic acid of polyoma virus. Virology. 1960;12:185. doi.org/10.1016/0042-6822(60)90193-8
	Dulbecco, R. and Freeman, G. Plaque production by the polyoma virus. Virology. 1959;8(3):396-397. doi: 10.1016/0042-6822(59)90043-1

Formulation

Coming soon!