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DMEM/F12 Medium with L-glutamine and HEPES, no phenol red, by Captivate Bio (case of 6 L)


Case of 6 for $75.00/1 L bottle.

DMEM/F12 Medium with L-glutamine and HEPES, no phenol red is a widely used basal medium formulated for the growth of various stem cell cultures including human pluripotent stem cells, induced pluripotent stem cells, human endothelial cells, and mouse embryonic stem cells.

[+] L-Glutamine, [+] HEPES, [+] Sodium Pyruvate, [-] Phenol Red

6 x 1000 mL

DMEM/F12 Medium with L-glutamine and HEPES, and added sodium pyruvate, no phenol red, by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. DMEM/F12 medium is a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. Cells cultured in DMEM/F12 with HEPES include mouse embryonic stem cells, epithelial cells, human endothelial cells, neural stem cells, human embryonic stem cells, mesenchymal stem cells, induced pluripotent stem cells, and hematopoietic stem cells.

DMEM/F12 is a serum-free, basal cell culture medium that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

This DMEM/F12 is modified as follows:

[+] L-Glutamine


[+] Sodium Pyruvate

[-] Phenol Red

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.


Catalog Number CB-DFHG02-CS6
Manufacturer / Country of Origin Captivate Bio / United States
Lot Number Included with shipment
Intended Use Mammalian cell culture, hPSCs, fibroblasts, human, mouse, rat, non-human primate, other cells
Details [+] L-Glutamine, [+] HEPES, [+] Sodium Pyruvate, [-] Phenol Red
Quantity 6 / case
Volume 1000 mL
Packaging Bottles
Form Liquid
Glutamine L-Glutamine
Phenol Red Indicator No Phenol Red
Osmolality 280-305 mOsm/kg
pH 7.0-7.4
Endotoxin < 1.0 EU/mL
Mycoplasma Negative
Sterility Sterile
Storage 2°C – 8°C (protect from light)
Shelf-Life 12 months from date of manufacture
Shipping Conditions Ambient temperature
Quality Statement This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.
Research Morton, H.J. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89-108. doi: 10.1007/BF02616112
Ham, R.G. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci. 1965;53(2):288-293. doi: 10.1073/pnas.53.2.288
Smith, J.D., Freeman, G., Vogt, M., and Dulbecco, R. The nucleic acid of polyoma virus. Virology. 1960;12:185.
Dulbecco, R. and Freeman, G. Plaque production by the polyoma virus. Virology. 1959;8(3):396-397. doi: 10.1016/0042-6822(59)90043-1


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