DMEM/F12 Medium with L-glutamine and phenol red, no HEPES, by Captivate Bio (case of 6 or 12)

DMEM/F12 Medium with L-glutamine and phenol red, and added sodium pyruvate, no HEPES, by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. DMEM/F12 is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells, including hPSCs, MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F12 is a serum-free, basal medium that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

This DMEM/F12 is modified as follows:

[+] L-Glutamine

[+] Phenol Red

[+] Sodium Pyruvate

[-] HEPES

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.

Specifications

Catalog Number	6 x 500 mL: CB-DF1201-CS6; 12 x 500 mL: CB-DF1201-CS12
Manufacturer / Country of Origin	Captivate Bio / United States
Lot Number	Included with shipment
Intended Use	Mammalian cell culture, organoids, 3D cultures, stem cells, hPSCs, iPSCs, hESCs, fibroblasts, human, mouse, rat, non-human Primate
Details	[+] L-Glutamine, [+] Phenol Red, [+] Sodium Pyruvate, [-] HEPES
Quantity	6 or 12 per case
Volume	500 mL
Packaging	Bottles
Form	Liquid
Glutamine	L-Glutamine
HEPES Buffer	No HEPES
Phenol Red	With Phenol Red
Osmolality	290-330 mOsm/kg
pH	7.0-7.4
Mycoplasma	Negative
Sterility	Sterile
Storage	2°C – 8°C (protect from light)
Shelf-Life	12 months from date of manufacture
Shipping Conditions	Ambient temperature
Quality Statement	This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.
Research	Morton, H.J. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89.
	Ham, R.G. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad.Sci. 1965;53:288.
	Smith, J.D., et al. The nucleic acid of polyoma virus. Virology. 1960;12:185.
	Dulbecco, R., et al. Plaque formation by the polyoma virus. Virology. 1959;8:396.

Formulation

Components	Molecular Weight	mg/L	mM
Amino Acids
Glycine	75	18.75	0.250000
L-Alanine	89	4.45	0.050000
L-Arginine hydrochloride	211	147.5	0.699052
L-Asparagine-H2O	150	7.5	0.050000
L-Aspartic acid	133	6.65	0.050000
L-Cysteine hydrochloride-H2O	176	17.56	0.099773
L-Cystine 2HCl	313	31.29	0.099968
L-Glutamic Acid	147	7.35	0.050000
L-Glutamine	146	365	2.500000
L-Histidine hydrochloride-H2O	210	31.48	0.149905
L-Isoleucine	131	54.47	0.415802
L-Leucine	131	59.05	0.450763
L-Lysine hydrochloride	183	91.25	0.498634
L-Methionine	149	17.24	0.115705
L-Phenylalanine	165	35.48	0.215030
L-Proline	115	17.25	0.150000
L-Serine	105	26.25	0.250000
L-Threonine	119	53.45	0.449160
L-Tryptophan	204	9.02	0.044216
L-Tyrosine disodium salt dihydrate	261	55.79	0.213755
L-Valine	117	52.85	0.451709
Vitamins
Biotin	244	0.0035	0.000014
Choline chloride	140	8.98	0.064143
D-Calcium pantothenate	477	2.24	0.004696
Folic Acid	441	2.65	0.006009
Niacinamide	122	2.02	0.016557
Pyridoxine hydrochloride	206	2.013	0.009772
Riboflavin	376	0.219	0.000582
Thiamine hydrochloride	337	2.17	0.006439
Vitamin B12	1355	0.68	0.000502
i-Inositol	180	12.6	0.070000
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111	116.6	1.050450
Cupric sulfate (CuSO4-5H2O)	250	0.0013	0.000005
Ferric Nitrate (Fe(NO3)3″9H2O)	404	0.05	0.000124
Ferric sulfate (FeSO4-7H2O)	278	0.417	0.001500
Magnesium Chloride (anhydrous)	95	28.64	0.301474
Magnesium Sulfate (MgSO4) (anhyd.)	120	48.84	0.407000
Potassium Chloride (KCl)	75	311.8	4.157333
Sodium Chloride (NaCl)	58	6995.5	120.612070
Sodium Phosphate dibasic (Na2HPO4) anhydrous	142	71.02	0.500141
Sodium Phosphate monobasic (NaH2PO4-H2O)	138	62.5	0.452899
Zinc sulfate (ZnSO4-7H2O)	288	0.432	0.001500
Other Components
D-Glucose (Dextrose)	180	3151	17.505556
Hypoxanthine Na	159	2.39	0.015031
Linoleic Acid	280	0.042	0.000150
Lipoic Acid	206	0.105	0.000510
Phenol Red	376.4	8.1	0.021520
Putrescine 2HCl	161	0.081	0.000503
Sodium Pyruvate	110	55	0.500000
Thymidine	242	0.365	0.001508
Sodium Bicarbonate	84	2438	29.021391

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