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DMEM/F12 Medium with L-glutamine and phenol red, no HEPES, by Captivate Bio

$270.00$435.00

DMEM/F12 Medium with L-glutamine and phenol red, no HEPES is a widely used basal medium formulated for the growth of mammalian cells, including MDCK, glial cells, fibroblasts, endothelial cells, stem cells, hPSC, iPSCs, and hESCs.

[+] L-Glutamine, [+] Phenol Red, [+] Sodium Pyruvate, [-] HEPES

500 mL

DMEM/F12 Medium with L-glutamine and phenol red, and added sodium pyruvate, no HEPES, by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. DMEM/F12 is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells, including hPSCs, MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F12 is a serum-free, basal medium that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

This DMEM/F12 is modified as follows:

[+] L-Glutamine

[+] Phenol Red

[+] Sodium Pyruvate

[-] HEPES

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.

Specifications

Catalog Number 6 x 500 mL: CB-DF1201-CS6; 12 x 500 mL: CB-DF1201-CS12
Manufacturer / Country of Origin Captivate Bio / United States
Lot Number Included with shipment
Intended Use Mammalian cell culture, organoids, 3D cultures, stem cells, hPSCs, iPSCs, hESCs, fibroblasts, human, mouse, rat, non-human Primate
Details [+] L-Glutamine, [+] Phenol Red, [+] Sodium Pyruvate, [-] HEPES
Quantity 6 or 12 per case
Volume 500 mL
Packaging Bottles
Form Liquid
Glutamine L-Glutamine
HEPES Buffer No HEPES
Phenol Red With Phenol Red
Osmolality 290-330 mOsm/kg
pH 7.0-7.4
Mycoplasma Negative
Sterility Sterile
Storage 2°C – 8°C (protect from light)
Shelf-Life 12 months from date of manufacture
Shipping Conditions Ambient temperature
Quality Statement This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.
Research Morton, H.J. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89.
Ham, R.G. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad.Sci. 1965;53:288.
Smith, J.D., et al. The nucleic acid of polyoma virus. Virology. 1960;12:185.
Dulbecco, R., et al. Plaque formation by the polyoma virus. Virology. 1959;8:396.

Formulation

Components Molecular Weight mg/L mM
Amino Acids
Glycine 75 18.75 0.250000
L-Alanine 89 4.45 0.050000
L-Arginine hydrochloride 211 147.5 0.699052
L-Asparagine-H2O 150 7.5 0.050000
L-Aspartic acid 133 6.65 0.050000
L-Cysteine hydrochloride-H2O 176 17.56 0.099773
L-Cystine 2HCl 313 31.29 0.099968
L-Glutamic Acid 147 7.35 0.050000
L-Glutamine 146 365 2.500000
L-Histidine hydrochloride-H2O 210 31.48 0.149905
L-Isoleucine 131 54.47 0.415802
L-Leucine 131 59.05 0.450763
L-Lysine hydrochloride 183 91.25 0.498634
L-Methionine 149 17.24 0.115705
L-Phenylalanine 165 35.48 0.215030
L-Proline 115 17.25 0.150000
L-Serine 105 26.25 0.250000
L-Threonine 119 53.45 0.449160
L-Tryptophan 204 9.02 0.044216
L-Tyrosine disodium salt dihydrate 261 55.79 0.213755
L-Valine 117 52.85 0.451709
Vitamins
Biotin 244 0.0035 0.000014
Choline chloride 140 8.98 0.064143
D-Calcium pantothenate 477 2.24 0.004696
Folic Acid 441 2.65 0.006009
Niacinamide 122 2.02 0.016557
Pyridoxine hydrochloride 206 2.013 0.009772
Riboflavin 376 0.219 0.000582
Thiamine hydrochloride 337 2.17 0.006439
Vitamin B12 1355 0.68 0.000502
i-Inositol 180 12.6 0.070000
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 116.6 1.050450
Cupric sulfate (CuSO4-5H2O) 250 0.0013 0.000005
Ferric Nitrate (Fe(NO3)3″9H2O) 404 0.05 0.000124
Ferric sulfate (FeSO4-7H2O) 278 0.417 0.001500
Magnesium Chloride (anhydrous) 95 28.64 0.301474
Magnesium Sulfate (MgSO4) (anhyd.) 120 48.84 0.407000
Potassium Chloride (KCl) 75 311.8 4.157333
Sodium Chloride (NaCl) 58 6995.5 120.612070
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 71.02 0.500141
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 62.5 0.452899
Zinc sulfate (ZnSO4-7H2O) 288 0.432 0.001500
Other Components
D-Glucose (Dextrose) 180 3151 17.505556
Hypoxanthine Na 159 2.39 0.015031
Linoleic Acid 280 0.042 0.000150
Lipoic Acid 206 0.105 0.000510
Phenol Red 376.4 8.1 0.021520
Putrescine 2HCl 161 0.081 0.000503
Sodium Pyruvate 110 55 0.500000
Thymidine 242 0.365 0.001508
Sodium Bicarbonate 84 2438 29.021391

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