DMEM, high glucose, with L-glutamine and phenol red, no HEPES, by Captivate Bio (case of 6)

$150.00

Case of 6 for $25.00/bottle.

DMEM, high glucose, with L-glutamine and phenol red, no HEPES is formulated for the growth of mammalian cells, including fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

[+] High Glucose, [+] L-Glutamine, [+] Phenol Red, [-] HEPES

6 x 500 mL

DMEM, high glucose, with L-glutamine and phenol red, no HEPES, by Captivate Bio is formulated for superior quality, reliability, and consistency. Dulbecco’s Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains high concentrations of amino acids and vitamins. The original culture media formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Cells successfully cultured in DMEM cell growth medium include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.  

This DMEM is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. DMEM contains no proteins, lipids, or growth factors and requires supplementation, commonly with 10% Fetal Bovine Serum (FBS) or 5-10% human platelet lysate (HPL). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH. 

This DMEM is modified as follows: 

[+] High Glucose 

[+] L-Glutamine 

[+] Phenol Red 

[-] HEPES 

cGMP Manufacturing 

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only. 

Catalog Number

CB-DMEM01-CS6

Manufacturer / Country of Origin

Captivate Bio / United States

Lot Number

Included with shipment

Intended Use

Primary fibroblasts, neurons, glial cells, HUVECs, mouse ES cells, human pluripotent cells, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

Details

[+] High Glucose, [+] L-Glutamine, [+] Phenol Red, [-] HEPES

Quantity

6 / case

Volume

500 mL

Packaging

Bottles

Form

Liquid

Glutamine

L-Glutamine

HEPES Buffer

No HEPES

Phenol Red Indicator

With Phenol Red

Osmolality

270-366 mOsm/kg

pH

7.0-7.4

Endotoxin

< 1.0 EU/mL

Mycoplasma

Negative

Sterility

Sterile

Storage

2°C – 8°C (protect from light)

Shelf-Life

12 months from date of manufacture

Shipping Conditions

Ambient temperature

Quality Statement

This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.

Contact us for the technical documents on this product.

Components

mg/L

Amino Acids

Glycine

30

L-Arginine HCl

84

L-Cystine 2HCl

62.6

L-Glutamine

584

L-Histidine HCl H2O

42

L-Isoleucine

105

L-Leucine

105

L-Lysine HCl

146

L-Methionine

30

L-Phenylalanine

66

L-Serine

42

L-Threonine

95

L-Tryptophan

16

L-Tyrosine 2Na 2H2O

103.79

L-Valine

94

Vitamins

Choline chloride

4

Folic Acid

4

Myo-Inositol

7.2

Niacinamide

4

Calcium D-pantothenate

4

Pyridoxine HCl

4

Riboflavin

0.4

Thiamine HCl

4

Inorganic Salts

CaCl2 2H2O

265

Fe(NO3)3 9H2O

0.1

MgSO4 (anhydrous)

97.7

KCl

400

NaHCO3

1500

NaCl

6400

Sodium Phosphate NaH2PO4 (anhydrous)

109

Other Components

D-Glucose

4500

Phenol Red, Sodium Salt

15

Sodium Pyruvate

110

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