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GradientEZ 3D Cell Migration System by Lena Biosciences


Versatile scaffold system used for 3D cell migration and invasion assays


GradientEZ by Lena Biosciences is a versatile system used for 3D cell migration and invasion assays, which enables cells to migrate in three dimensions as they do in living tissues. It is composed of completely inert, transparent, and hydrophilic glass microfibers. Its design enables parallel testing of multiple modulators of cell motility or invasion, drug gradients, or drugs in different concentrations, in a single well. This allows researchers to model and study gradient-dependent pathological conditions in 3D including cancer, inflammatory, infectious and autoimmune diseases. It also enables them to test therapeutic strategies in these models in vitro. Cells can be isolated for quantitative results or fluorescently labeled and imaged in real time.

GradientEZ is simple to use yet enables researchers to study even higher order 3D cell processes in vitro, such as metastases. It is compatible with all cell types and extracellular matrices. Examples of cells used in GradientEZ include primary human neutrophils, T cells, B cells, cancer cells, and normal brain, bone, liver, and lung cells. Choose between the 6-lobe or 6-petal design. The 6-petal system fits into 24-well plates and larger dishes and is ideally suited for prolonged nerve regeneration studies. The 6-lobe system fits into 12-well plates and larger dishes.


  • Permits the generation of complex and reproducible gradients of soluble factors and substrate-bound molecules
  • Supports co- and counter-gradients of multiple test compounds applied in cell invasion and migration assays, chemotaxis and chemo-invasion assays, and angiogenesis assays
  • Enables concurrent testing of drug combination strategies
  • Enables the testing and study of metastases in vitro

How it works:

  • Simply pipet cells in the center and add test agents to petals or lobes.
  • Alternatively, to test metastatic potential of cancer cells in vitro, add invasive cells to the center and normal cells (e.g. brain, lung, liver, bone, adrenal, etc.) to lobes and track invasion to begin to explain where they might metastasize in vivo.
  • For parallel testing of both attractants and repellents, add them to opposing lobes to monitor if inflammatory cells could be directed more efficiently from the central site of “chronic inflammation.”
  • Fluorescently labeled cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell migration studies using fluorescence microscopy.


Pack of 12. Sterile.

Ships within 1 week.

Additional information

GradientEZ design



Catalog Number LB-2015-06 / LB-2025-06
Lot Number Included with shipment
Country of Manufacture USA
Quantity Pack of 12
Packaging 1 package; 6 each per sterile section
Material Hydrophilic glass microfibers
Sterility Sterile
Features 6-lobe or 6-petal design; 400 µm thick
Compatibility 6-lobe: 12-well or larger; 6-petal: 24-well or larger
Shelf Life 2 years from receipt
Storage Room temperature
Shipping Info Ships from manufacturer [standard shipping within 1 week]
Product Use This product is for research use only. It is not approved for use in humans, animals, or for diagnostic procedures.
References Nck-associated Protein 1 Associates with HSP90 to Drive Metastasis in Human Non-Small-Cell Lung Cancer. Xiong Y, He L, Shay C, Lang L, Loveless J, Yu J, Chemmalakuzhy R, Jiang H, Liu M, Teng Y.. J Exp Clin Cancer Res. 2019, 38(1):122. DOI: 10.1186/s13046-019-1124-0.

Cytochrome P450 enzyme activity is enhanced in hepatocytes grown using a perfused 3D cell culture drug screening system. Shoemaker J.T. and Vukasinovic J. American Association for Cancer Research 2017. Volume 77, Issue 13 Supplement, pp. 4080. DOI: 10.1158/1538-7445.AM2017-4080.

Progress towards the development of a fully humanised 3D cell culture model of HER2+ breast cancer. Sophie Roberts, Danah Al’Qallaf, Sally A. Peyman, Darren Tomlinson, J.T. Shoemaker, Jelena Vukasinovic and Valerie Speirs. Breast Cancer Res Treat (2018) 167: 309. DOI: 10.1007/s10549-017-4585-x.

United States Patent No. 9,334,473

Technical Resources

LB-2015-06 Technical Data Sheet
LB-2025-06 Technical Data Sheet

All files supplied as PDFs