Human umbilical vein endothelial cells (HUVECs) serve as a model system for the study of endothelial cell function and disease, and are commonly used in studies involving angiogenesis, homeostasis, inflammatory disorders, atherosclerosis, cancer, and cardiovascular-related diseases.
HUVECs were isolated from the umbilical cord vein of a single donor (female offspring). Cells are cultured, passaged for purity, cryopreserved, and delivered frozen. Each vial contains 1 x 106 cells in 1 mL volume. Cells are characterized by flow cytometry for CD31, CD144, CD146, and CD45. HUVECs are negative for mycoplasma, yeast, fungi, and bacteria. HIV-1, HIV-2, and HBV are not detected. Cells are assured to further culture using the protocol provided by JangoCell.
Human umbilical vein endothelial cells (HUVECs) are also known as: Primary human umbilical vein endothelial cells, HUV-EC-C, HUVEC cells, and huVECs
Product Use
This cell product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.
Human Material Precaution
Tissues used for cell isolation were acquired with informed consent and adhere to HIPAA standards for human privacy protection. All human biological material, including human cells, should always be handled as potentially infectious using at least Biosafety Level 2 precautions; we recommend that appropriate safety procedures be followed, using the same biosafety protocol used with known infectious material. Viral testing cannot prove the absence of a latent viral genome.
JangoCell Statement on Passage Number
While passage number is the de facto metric used for determining cell proliferation capacity, the actual capacity is dependent upon the total number of cell divisions (or population doubling number (PDN)) that a particular cell type can undergo. This can be illustrated with a simple example. Since stem cells are typically passaged upon reaching confluence, cells grown at low confluence in a flask or in a large vessel such as a bioreactor (large surface area to cell number) will require many more doublings to reach confluence before they are ‘passaged’ than cells seeded at high confluence. This can result in low passage numbers, but a cell population with more limited capacity for further cell division. Therefore, passage number is not the most reliable means of determining future cell proliferation capacity after purchase.
For these reasons, and to provide transparency concerning our cell products, JangoCell will express future cell division capacity, or the number of times that our cells will be able to divide and double in number after purchase (after thaw), in terms of population doubling number (PDN). We will express the current cell division number, or the number of times that our cells have already divided and doubled in number before cryopreservation, in terms of population doubling level (PDL). JangoCell’s primary stem cells when plated at ~20% confluency will display a PDN between 30-40 when cultured using the protocol recommended by JangoCell for each specific cell type.
Please see our page on passage number under Technical Resources for more information.
Contact us for the technical documents on this product.
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