MEM α Medium with nucleosides and L-glutamine, no phenol red

$47.50$72.50

MEM α (Minimum Essential Medium with alpha modification) with nucleosides and L-glutamine, no phenol red is a widely used basal medium formulated for the growth of a variety of mammalian cell types

[+] Ribonucleosides, [+] Deoxyribonucleosides, [+] L-glutamine, [-] phenol red

MEM α (Minimum Essential Medium with alpha modification) with nucleosides and L-glutamine, no phenol red by JangoCell is a sterile, serum-free basal cell culture medium with added ribonucleosides, deoxyribonucleosides, and L-glutamine. MEM α is a widely used basal medium, formulated for the growth of a variety of mammalian cell types, including but not limited to keratinocytes, primary rat astrocytes, human melanoma cells, mesenchymal stem cells, and cancer cells. This cell culture media is typically used for cells grown in monolayers but is appropriate for both suspension and adherent mammalian cell culture with additional supplementation. Alpha MEM media has been used across a broad range of cell culture applications and experimental studies in reproductive biology, gene expression studies, and transplant and regenerative medicine research.

MEM α is a modification of Eagles Minimum Essential Medium that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM alpha with nucleosides and L-glutamine contains no proteins, no lipids, and no growth factors (animal origin-free), so supplementation of these factors may be required for your specific cell type; 10% Fetal Bovine Serum (FBS) is often used. This Alpha MEM liquid culture media is made with Earle’s salts and requires a 5-10% CO2 environment to maintain physiological pH. JangoCell’s MEM α is manufactured under strict industry standards.

Phenol Red is known to interfere with certain biochemical reactions and fluorescent dyes. Phenol red free media circumvents these issues, allowing no confusion with data interpretation.

MEM α (Minimum Essential Medium with alpha modification) is also known as: Minimum Essential Medium α, Alpha MEM, MEM-Alpha, MEM Alpha, Minimum Essential Medium Alpha Modification, MEM Alpha Modification, α MEM, αMEM, α-MEM, and MEM-α.

 

This MEM α is modified as follows:

[+] Ribonucleosides

[+] Deoxyribonucleosides

[+] L-glutamine

[-] Phenol red

 

Product Use

This product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.

Additional information

Volume

500 mL, 1000 mL

Catalog Number

500 mL: 104524-500ML; 1000 mL: 104524-1L

Country of Origin

USA

Lot Number

Included with shipment

Intended Use

Mammalian cell types; keratinocytes, primary rat astrocytes, human melanoma cells, mesenchymal stem cells, and cancer cells

Classification

Animal Origin-free

Concentration

1X

Details

[+] Ribonucleosides, [+] Deoxyribonucleosides, [+] L-glutamine, [-] phenol red

Volume

500 mL or 1000 mL

Packaging

Bottles

Form

Liquid

Glutamine

L-glutamine

HEPES Buffer

No HEPES

Phenol Red Indicator

Without Phenol Red

Osmolality

280 – 330 mM/Kg

pH

7.0 – 7.4

Endotoxin

< 0.1 EU/mL

Mycoplasma

Negative

Sterility

Sterile

Storage

Refrigerate at 2°C – 8°C (protect from light)

Shelf-Life

18 months from date of manufacture

Shipping Conditions

Ambient temperature; Ground Shipping. Refrigerate on arrival

Also Known As

Minimum Essential Medium α, Alpha MEM, MEM-Alpha, MEM Alpha, Minimum Essential Medium Alpha Modification, MEM Alpha Modification, α MEM, αMEM, α-MEM, and MEM-α

Product Use

This product is intended for laboratory research purposes only. Not approved for use in human subjects or for clinical, diagnostic, or therapeutic use in animals or humans.

References

Coelho MJ, Cabral AT, and Fernandes MH. Human bone cell cultures in biocompatibility testing. Part I: osteoblastic differentiation of serially passaged human bone marrow cells cultured in α-MEM and in DMEM. Biomaterials. 2000; 21(11): 1087-1094. doi: 10.1016/S0142-9612(99)00284-7.

Dai X, Cheng H, Bai Z, and Li J. Breast cancer cell line classification and its relevance with breast tumor subtyping. Journal of Cancer. 2017; 8(16): 3131-3141. doi: 10.7150/jca.18457.

Morgan A, Babu D, Reiz B, et al. Caution for the routine use of phenol red – It is more than just a pH indicator. Chemico-Biological Interactions. 2019; 310: 108739. doi: 10.1016/j.cbi.2019.108739.

Vireque AA, Camargo LSA, Serapião RV, et al. Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. Theriogenology. 2009; 71(4): 620-627. doi: 10.1016/j.theriogenology.2008.09.028.

Watchrarat K, Korchunjit W, Buranasinsup S, et al. MEM α promotes cell proliferation and expression of bone marrow derived equine mesenchymal stem cell gene markers but depresses differentiation gene markers. Journal of Equine Veterinary Science. 2017; 50: 8-14. doi: 10.1016/j.jevs.2016.10.017.

Wun SA, Wun CC, and Grunert GM. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. mouse embryo study. Journal of Assisted Reproduction and Genetics. 1994; 11: 303-307.  doi: 10.1007/BF02215717.

Contact us for the technical documents on this product.

Contact us for the formulation.

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