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Mesenchymal Stem Cell (MSC) Medium Kit by Applied Biological Materials (abm)

$225.00

The Mesenchymal Stem Cell Medium Kit is formulated and optimized to support the derivation of bone marrow mesenchymal stem cells and promote long-term culture of these cells.
1 kit; 500 mL

SKU ABM-TM022 Category Tags , , , , ,

Description

The Mesenchymal Stem Cell Medium Kit by abm is formulated and optimized to not only support the derivation of bone marrow mesenchymal stem cells (MSCs), but also promote long-term culture of these cells. This medium has been tested to support long-term robust proliferation of MSCs from mice, rats, and humans. Customer feedback shows that this proprietary medium is more cost-effective than homemade media, while the quality is better than kits from other leading suppliers.

The Mesenchymal Stem Cell Medium Kit has 2 components, the basal medium and the growth supplements to allow flexibility for daily cell culture applications.

Each lot is tested for the ability to promote proliferation of mesenchymal stem cells. All components are endotoxin free and negative for bacteria, yeast, and fungi.

Supplied by Applied Biological Materials (abm). Usually ships in 3 business days.

Specifications

Catalog Number ABM-TM022
Manufacturer Applied Biological Materials (abm)
Lot Number Included with shipment
Quantity Includes 500 mL basal medium and MSC Medium Growth Supplements
Storage Store Basal Medium at 2-8°C and Growth Supplements at -20°C.
Shipping Info Ice packs; Usually ships in 3 business days.
Product Use This product is for research use only. It is not approved for use in humans, animals, or for diagnostic procedures.
References Meng, X et al. “Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor” Cell Res. 23(5:658-672 (2013). DOI: 10.1038/cr.2013.40. PubMed: 23478301.

Chen, W et al. “PDGFB-based stem cell gene therapy increases bone strength in the mouse” PNAS 29:E3893-E3900 (2015). DOI: 10.1073/pnas.1501759112.

Baylink, D et al. “SYSTEMIC AND LOCAL EX VIVO GENE THERAPY OF THE SKELETON” United States Patent Application 20160120944: (2016).

FAQs

What is the ratio to add the supplements to the media?
Add 5 ml of the concentrated supplement to 45 ml MEM medium.

What is the shelf life after the supplements are added to the media?
The shelf-life is at least 1 month at 4˚C. After one month of storage at 4˚C, there is no obvious change in quality.

What is the shelf life of the basal media and supplements (separately)?
If the basal media is stored at 4˚C, it can last at least a year. If the supplements are stored at 4˚C, it can last 1-2 months. If the supplements are stored at -20˚C, it can last approximately one year as well. Multiple freezing and thawed is not encouraged and we recommend making aliquots before freezing. Based on our experience, there is no obvious change in quality after 1-2 freeze-thaw cycles.

I am using the MSC medium kit to culture the MSCs. I have a question about whether the MSC medium contains serum. Can you tell me the components of the medium?
The medium contains low-level serum and multiple supplements including growth factors. Generally, MSCs grow better in fibronectin-precoated non-TC plates and in hypoxia.

Does this product contain Ammonium metavanadate?
No