The Mesenchymal Stem Cell Medium Kit by abm is formulated and optimized to not only support the derivation of bone marrow mesenchymal stem cells (MSCs), but also promote long-term culture of these cells. This medium has been tested to support long-term robust proliferation of MSCs from mice, rats, and humans. Customer feedback shows that this proprietary medium is more cost-effective than homemade media, while the quality is better than kits from other leading suppliers.
The Mesenchymal Stem Cell Medium Kit has 2 components, the basal medium and the growth supplements to allow flexibility for daily cell culture applications.
Each lot is tested for the ability to promote proliferation of mesenchymal stem cells. All components are endotoxin free and negative for bacteria, yeast, and fungi.
Supplied by Applied Biological Materials (abm). Usually ships in 3 business days.
What is the ratio to add the supplements to the media?
Add 5 ml of the concentrated supplement to 45 ml MEM medium.
What is the shelf life after the supplements are added to the media?
The shelf-life is at least 1 month at 4˚C. After one month of storage at 4˚C, there is no obvious change in quality.
What is the shelf life of the basal media and supplements (separately)?
If the basal media is stored at 4˚C, it can last at least a year. If the supplements are stored at 4˚C, it can last 1-2 months. If the supplements are stored at -20˚C, it can last approximately one year as well. Multiple freezing and thawed is not encouraged and we recommend making aliquots before freezing. Based on our experience, there is no obvious change in quality after 1-2 freeze-thaw cycles.
I am using the MSC medium kit to culture the MSCs. I have a question about whether the MSC medium contains serum. Can you tell me the components of the medium?
The medium contains low-level serum and multiple supplements including growth factors. Generally, MSCs grow better in fibronectin-precoated non-TC plates and in hypoxia.
Does this product contain Ammonium metavanadate?