Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stem cells with a high capacity for self-renewal. They can develop into specialized cells of the body such as chondrocytes, osteoblasts and adipocytes. Bone marrow-derived MSCs can also differentiate into nerve cells, heart muscle cells, liver cells and endothelial cells. Their easy accessibility, exceptional genomic stability, and their capacity for tissue repair with few immunological and ethical issues make them the favorable cell type for experimental and clinical use. Many trials are underway using bone marrow mesenchymal stem cells to understand and treat osteoarthritis, sepsis, autoimmune diseases, graft versus host disease, Crohn’s disease, multiple sclerosis, systemic lupus erythematosus and systemic sclerosis.
These rat bone marrow MSCs are isolated from neonatal (day 18) male Fischer rat leg bone. The cells are cultured, passaged for purity, cryopreserved, and delivered frozen. Each vial contains 1 x 106 cells in 1 mL volume. These rat bone marrow-derived stem cells are characterized by flow cytometry and are negative for mycoplasma, yeast, fungi, and bacteria. Cells are assured to further culture using the protocol provided by JangoCell.
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are also known as: BMMSCs, BMSCs, bone marrow-derived mesenchymal stromal cells, bone marrow stromal stem cells, bone marrow cells, bone mesenchymal stem cells, MSC cells, MSC stem cells, adult mesenchymal stem cells, and adult stem cells.
JangoCell Statement on Passage Number
While passage number is the de facto metric used for determining cell proliferation capacity, the actual capacity is dependent upon the total number of cell divisions (or population doubling number (PDN)) that a particular cell type can undergo. This can be illustrated with a simple example. Since stem cells are typically passaged upon reaching confluence, cells grown at low confluence in a flask or in a large vessel such as a bioreactor (large surface area to cell number) will require many more doublings to reach confluence before they are ‘passaged’ than cells seeded at high confluence. This can result in low passage numbers, but a cell population with more limited capacity for further cell division. Therefore, passage number is not the most reliable means of determining future cell proliferation capacity after purchase.
For these reasons, and to provide transparency concerning our cell products, JangoCell will express future cell division capacity, or the number of times that our cells will be able to divide and double in number after purchase (after thaw), in terms of population doubling number (PDN). We will express the current cell division number, or the number of times that our cells have already divided and doubled in number before cryopreservation, in terms of population doubling level (PDL). JangoCell’s primary stem cells when plated at ~20% confluency will display a PDN between 30-40 when cultured using the protocol recommended by JangoCell for each specific cell type.
Please see our page on passage number under Technical Resources for more information.
Contact us for the technical documents on this product.
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