Adipose-derived stem cells (ADSCs) are multipotent cells with a high capacity for self-renewal. They are ideal for regenerative therapy applications. Adipose tissue-derived stem cells can differentiate into specialized cells of the body such as osteocytes, adipocytes, neural cells, vascular endothelial cells, cardiomyocytes, pancreatic β-cells, and hepatocytes. Their easy accessibility, low immunogenicity and their ability to secrete trophic factors, cytokines and chemokines make them the favorable cell type for experimental and clinical use. Adipose-derived adult stem cells are also demonstrated to have proangiogenic, immunomodulatory, anti-apoptotic, anti-inflammatory, and anti-scarring effects. Many trials are underway to understand the potential of these cells to be used for a wide range of clinical conditions including skeletal tissue repair, ischemic injuries, myocardial infarction, soft tissue regeneration and immune disorders such as lupus, arthritis, Crohn’s disease, multiple sclerosis, diabetes mellitus, and graft-versus-host disease.
These Sprague Dawley rat adipose-derived stem cells are isolated from old adult (21 month) female Sprague Dawley rat adipose tissue. The cells are cultured, passaged for purity, cryopreserved, and delivered frozen. Each vial contains 1 x 106 cells in 1 mL volume. These rat adipose mesenchymal stem cells are characterized by flow cytometry for CD90, CD105, CD44, CD29, CD45, and CD34 and are negative for mycoplasma, yeast, fungi, and bacteria. Cells are assured to further culture using the protocol provided by JangoCell.
Adipose-derived stem cells (ADSC) are also known as: AD-MSC, ADMSC, ASC, ASCS, adipose-derived mesenchymal stem cells, adipose-derived MSC, adipose-derived adult stem cells, adipose-derived stromal cells, and adipose stem cells.
JangoCell Statement on Passage Number
While passage number is the de facto metric used for determining cell proliferation capacity, the actual capacity is dependent upon the total number of cell divisions (or population doubling number (PDN)) that a particular cell type can undergo. This can be illustrated with a simple example. Since stem cells are typically passaged upon reaching confluence, cells grown at low confluence in a flask or in a large vessel such as a bioreactor (large surface area to cell number) will require many more doublings to reach confluence before they are ‘passaged’ than cells seeded at high confluence. This can result in low passage numbers, but a cell population with more limited capacity for further cell division. Therefore, passage number is not the most reliable means of determining future cell proliferation capacity after purchase.
For these reasons, and to provide transparency concerning our cell products, JangoCell will express future cell division capacity, or the number of times that our cells will be able to divide and double in number after purchase (after thaw), in terms of population doubling number (PDN). We will express the current cell division number, or the number of times that our cells have already divided and doubled in number before cryopreservation, in terms of population doubling level (PDL). JangoCell’s primary stem cells when plated at ~20% confluency will display a PDN between 30-40 when cultured using the protocol recommended by JangoCell for each specific cell type.
Please see our page on passage number under Technical Resources for more information.
Contact us for the technical documents on this product.
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