RPMI 1640 Medium with L-glutamine and phenol red, no HEPES, by Captivate Bio (case of 6)

$150.00

Case of 6 for $25.00/bottle.

RPMI 1640 Medium was originally developed for culturing human leukocytes and other blood cells, and is now used for a wide range of mammalian cells in suspension and monolayer cell culture, including HeLa, Jurkat, PC-12, PBMC, astrocytes, and carcinomas.

[+] L-Glutamine, [+] Phenol Red, [-] HEPES

6 x 500 mL

RPMI 1640 Medium with L-glutamine and phenol red, no HEPES, by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. Roswell Park Memorial Institute (RPMI) 1640 Medium was originally developed for culturing human leukocytes and other blood cells and is now used for a range of mammalian cells in suspension and monolayer cell culture, including HeLa, Jurkat, PC-12, PBMC, astrocytes, and carcinomas.

This RPMI 1640 medium is modified as follows:

[+] L-Glutamine

[+] Phenol Red

[-] HEPES

Using RPMI 1640

RPMI 1640 Medium is a unique classical cell culture media formulation as it contains a reducing agent called glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimum Essential Medium (EMEM) or Dulbecco’s Modified Eagle Medium (DMEM). This medium contains no proteins, lipids, or growth factors, and therefore requires supplementation with serum like FBS or platelet lysate.

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.

Catalog Number

CB-RPMI01-CS6

Manufacturer / Country of Origin

Captivate Bio / United States

Lot Number

Included with shipment

Intended Use

Mammalian cell culture, suspension cell lines, human normal and neoplastic leukocytes, including unfixed specimens for use in flow cytometry, FISH, molecular and cytogenetics assays

Details

[+] L-Glutamine, [+] Phenol Red, [-] HEPES

Quantity

6 / case

Volume

500 mL

Packaging

Bottles

Form

Liquid

Glutamine

With L-Glutamine

Phenol Red Indicator

With Phenol Red

HEPES

No HEPES

pH

7.0-7.4

Osmolality

266-294 mOsm/kg

Mycoplasma

Negative

Sterility

Sterile

Storage

2°C – 8°C (protect from light)

Shelf-Life

12 months from date of manufacture

Shipping Conditions

Ambient temperature

Also Known As

RPMI medium, Roswell Park Memorial Institute medium, RPMI 1640, RPMI-1640 medium, RPMI-1640, and RPMI

Quality Statement

This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.

 

Contact us for the technical documents on this product.

Components

Molecular Weight

mg/L

mM

Amino Acids

Glycine

75

10

0.133333

L-Arginine-HCL

210.66

241.86

1.148106

L-Asparagine-H2O

152.12

56.82

0.373521

L-Aspartic acid

133

20

0.150376

L-Cystine

240.3

49.87

0.207532

L-Glutamic Acid

147

20

0.136054

L-Glutamine

146

292

2.000000

L-Histidine

155

20.27

0.130774

L-Hydroxyproline

131

20

0.152672

L-Isoleucine

131

50

0.381679

L-Leucine

131

50

0.381679

L-Lysine hydrochloride

183

40

0.218579

L-Methionine

149

15

0.100671

L-Phenylalanine

165

15

0.090909

L-Proline

115

20

0.173913

L-Serine

105

30

0.285714

L-Threonine

119

20

0.168067

L-Tryptophan

204

5

0.024510

L-Tyrosine disodium salt dihydrate

261

29

0.111111

L-Valine

117

20

0.170940

Vitamins

Biotin

244

0.2

0.000820

Choline chloride

140

3

0.021429

Calcium D-pantothenate

476.5

0.253

0.000531

Folic Acid

441

1

0.002268

Niacinamide

122

1

0.008197

Para-Aminobenzoic Acid

137

1

0.007299

Pyridoxine hydrochloride

206

1

0.004854

Riboflavin

376

0.2

0.000532

Thiamine hydrochloride

337

1

0.002967

Vitamin B12

1355

0.005

0.000004

i-Inositol

180

35

0.194444

Inorganic Salts

Calcium nitrate (Ca(NO3)2 4H2O)

236

100

0.423729

Magnesium Sulfate (MgSO4) (anhyd.)

120

48.84

0.407000

Potassium Chloride (KCl)

75

400

5.333334

Sodium Bicarbonate (NaHCO3)

84

2000

23.809525

Sodium Chloride (NaCl)

58

6000

103.448270

Sodium Phosphate dibasic (Na2HPO4) anhydrous

142

800

5.633803

Other Components

D-Glucose (Dextrose)

180

2000

11.111111

Glutathione (reduced)

307

1

0.003257

Phenol Red

376.4

5

0.013284

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