RPMI 1640 Medium with phenol red, no L-glutamine, no glucose, by Captivate Bio (case of 6)

$150.00

Case of 6 for $25.00/bottle.

RPMI 1640 Medium was originally developed for culturing human leukocytes and other blood cells, and is now used for a wide range of mammalian cells in suspension and monolayer cell culture, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. Without L-glutamine or glucose.

[+] Phenol Red, [+] Sodium Bicarbonate, [-] L-Glutamine, [-] Glucose, [-] HEPES

6 x 500 mL

RPMI 1640 Medium with phenol red and added sodium bicarbonate, no L-glutamine, no glucose, no HEPES by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell culture. Roswell Park Memorial Institute (RPMI) 1640 Medium was originally developed for the long-term culture of blood cells and the culture of normal and abnormal human leukocytes (e.g. neoplastic WBCs), and is now used as a general medium (with serum) for hybridoma cultures. RPMI 1640 medium has also been found suitable for a range of mammalian cell lines in suspension cell culture and monolayer cell culture, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. 

This RPMI 1640 medium is modified as follows: 

[+] Phenol Red 

[+] Sodium Bicarbonate 

[-] L-Glutamine 

[-] Glucose 

[-] HEPES 

Using RPMI 1640 

RPMI 1640 Medium is a unique classical cell culture media formulation as it contains a reducing agent called glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimum Essential Medium (EMEM) or Dulbecco’s Modified Eagle Medium (DMEM). This medium contains no proteins, lipids, or growth factors, and therefore requires supplementation with serum like FBS or platelet lysate. 

cGMP Manufacturing 

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only. 

RPMI 1640 Medium is also known as: RPMI medium, Roswell Park Memorial Institute medium, RPMI 1640, RPMI-1640 medium, RPMI-1640, and RPMI 

 

Catalog Number

CB-RPMN01-CS6

Manufacturer / Country of Origin

Captivate Bio / United States

Lot Number

Included with shipment

Intended Use

Suspension cell lines, human normal and neoplastic leukocytes, and a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas.

Details

[+] Phenol Red, [+] Sodium Bicarbonate, [-] L-Glutamine, [-] Glucose, [-] HEPES

Quantity

6 / case

Volume

500 mL

Packaging

Bottles

Form

Liquid

Glutamine

No Glutamine

Glucose

No Glucose

Phenol Red Indicator

With Phenol Red

HEPES

No HEPES

pH

7.0-7.4

Osmolality

260-300 mOsm/kg

Mycoplasma

Negative

Sterility

Sterile

Storage

2°C – 8°C (protect from light)

Shelf-Life

12 months from date of manufacture

Shipping Conditions

Ambient temperature

Also Known As

RPMI medium, Roswell Park Memorial Institute medium, RPMI 1640, RPMI-1640 medium, RPMI-1640, and RPMI

Quality Statement

This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.

References

Moore, G.E., Gerner, R.E., and Franklin, H.A. Culture of normal human leukocytes. JAMA. 1967;199(8):519-524. PMID 4960081

Contact us for the technical documents on this product.

Components

mg/L

Amino Acids

L-Arginine HCl

242

L-Asparagine

50

L-Aspartic Acid

20

L-Cystine

50

L-Glutamic Acid

20

Glycine

10

L-Histidine

15

L-Hydroxyproline

20

L-Isoleucine

50

L-Leucine

50

L-Lysine hydrochloride

40

L-Methionine

15

L-Phenylalanine

15

L-Proline

20

L-Serine

30

L-Threonine

20

L-Tryptophan

5

L-Tyrosine 2Na 2H2O

29

L-Valine

20

Vitamins

Biotin

0.2

D-Ca Pantothenate

0.25

Choline Chloride

3

Folic Acid

1

i-Inositol

35

Niacinamide

1

p-Aminobenzoic Acid (PABA)

1

Pyridoxine HCl

1

Riboflavin

0.2

Thiamine HCl

1

Vitamin B12

0.005

Inorganic Salts

Calcium nitrate (Ca(NO3)2 4H2O)

100

Potassium chloride (KCl)

400

Magnesium sulfate (MgSO4)

48.84

Sodium chloride (NaCl)

6000

Sodium bicarbonate (NaHCO3)

2000

Sodium Phosphate (Na2HPO4) (anhydrous)

800

Other Components

Glutathione (reduced)

1

Phenol Red

5

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