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RPMI 1640 Medium with phenol red, no L-glutamine, no glucose, by Captivate Bio

$150.00

RPMI 1640 Medium was originally developed for culturing human leukocytes and other blood cells, and is now used for a wide range of mammalian cells in suspension and monolayer cell culture, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. Without L-glutamine or glucose.

[+] Phenol Red, [+] Sodium Bicarbonate, [-] L-Glutamine, [-] Glucose, [-] HEPES

6 x 500 mL

RPMI 1640 Medium with phenol red and added sodium bicarbonate, no L-glutamine, no glucose, no HEPES by Captivate Bio is formulated for superior quality, reliability, and consistency for use in mammalian cell cultures. Roswell Park Memorial Institute (RPMI) 1640 Medium was originally developed for the long-term culture of blood cells and the culture of normal and abnormal human leukocytes (e.g. neoplastic WBCs), and is now used as a general medium (with serum) for hybridoma cultures. RPMI 1640 medium has also been found suitable for a range of mammalian cells in suspension and monolayer cell culture, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas.

This RPMI 1640 medium is modified as follows:

[+] Phenol Red

[+] Sodium Bicarbonate

[-] L-Glutamine

[-] Glucose

[-] HEPES

Using RPMI 1640

RPMI 1640 Medium is a unique classical cell culture media formulation as it contains a reducing agent called glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimum Essential Medium (EMEM) or Dulbecco’s Modified Eagle Medium (DMEM). This medium contains no proteins, lipids, or growth factors, and therefore requires supplementation with serum like FBS or platelet lysate.

cGMP Manufacturing

Captivate Bio products are produced in cGMP compliant and ISO-certified facilities which are designed to provide you with consistent, safe, and accurate manufacturing options that are essential to the success of your research. All Captivate Bio products are For Research Use or further manufacturing use only.

Specifications

Catalog Number

CB-RPMN01-CS6

Manufacturer / Country of Origin

Captivate Bio / United States

Lot Number

Included with shipment

Intended Use

Suspension cell lines, human normal and neoplastic leukocytes, and a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas.

Details

[+] Phenol Red, [+] Sodium Bicarbonate, [-] L-Glutamine, [-] Glucose, [-] HEPES

Quantity

6 / case

Volume

500 mL

Packaging

Bottles

Form

Liquid

Glutamine

No Glutamine

Glucose

No Glucose

Phenol Red Indicator

With Phenol Red

HEPES

No HEPES

pH

7.0-7.4

Osmolality

260-300 mOsm/kg

Mycoplasma

Negative

Sterility

Sterile

Storage

2°C – 8°C (protect from light)

Shelf-Life

12 months from date of manufacture

Shipping Conditions

Ambient temperature

Quality Statement

This product is for Research Use Only and is not intended for therapeutic or diagnostic use. Uses other than the labeled intended use may be a violation of federal law.

References

Moore, G.E., Gerner, R.E., and Franklin, H.A. Culture of normal human leukocytes. JAMA. 1967;199(8):519-524. PMID 4960081

Formulation

Coming soon!

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